|InterJournal Complex Systems, 1030
|Manuscript Number: |
Submission Date: 2004
|INTERACTION OF TUMOR CELLS AND IMMUNE SYSTEM: INTERINDUCTION OF APOPTOSIS BETWEEN TUMOR HEPATOCYTES AND SPLENOCYTES|
Homeostasis on the cellular, tissue and organism levels is a complex biological system, that is needed for normal development and function of organism and also reaction of organism on the unwanted factors – malignant transformation, infections, another damages. Interaction of tumor cells and immune system by means lymphocytes is one of form of homeostatic reactions. Heterogeneity of tumors for different characters increases during tumor progression and determines its malignancy. To reveal possible correlations between different characters, the method of clonal analysis of the tumor cell population is to be used. The goal of this study was to reveal effects of different degrees of DNA-polymorphisms of mouse hepatoma cells MH-22a on their capability for differentiation when growing in the eye anterior chamber (EAC) and on their ability to induce apoptosis of splenocytes at cocultivation, as well as on resistance to apoptosis produced by splenocytes. The DNA-polymorphism was revealed using RAPD-PCR with three random primers. The capability of tumor hepatocytes for differentiation was determined at their transplantation into the EAC of singenic mice C3HA. The capability for interinduction of apoptosis between tumor hepatocytes and singenic splenocytes was determined after their cocultivation in vitro. The presence of apoptosis was detected by the method of electrophoresis of low molecular DNA fractions, and in hepatocytes also by the method of clonal survival. It was shown that the clonal lines MH-22a with a high level of genetic variability were unable to differentiate when growing in EAC. They were stable to apoptosis induction by splenocytes, but could induce apoptosis of splenocytes. The basic population of tumor hepatocytes and clonal lines with a low level of genetic variability were differentiated at their growth in EAC and had capability for interinduction of apoptosis with singenic splenocytes.
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